16S 单样本分析报告

样本: demo

输入文件: /mnt/hdd/BAK_HDD2/test/demo/Result/demo.final.tsv

生成时间: 2026-05-21 11:11:40

1. 指标概览i汇总本样本的核心多样性指标。Observed Species 为检出的物种总数；Shannon 指数综合衡量物种丰富度与均匀度（值越大多样性越高）；Gini-Simpson 指数表示随机选取两个个体属于不同物种的概率；Top1 Abundance 为最优势物种的相对丰度；Other 为 Top N 之外物种的丰度合计；ASV Rows 为原始鉴定结果总行数。

Observed Species
76
Shannon
2.6606
Gini-Simpson
0.8986
Top1 Abundance
18.72%
Other
4.17%
ASV Rows
77

2. 质控结果（QC）i展示测序原始数据的质量控制结果，包括有效 reads 数量、平均读长及质量过滤情况，用于评估下游分析所用数据的可靠性。质控步骤通常包括去除低质量碱基、过滤短序列及去除接头序列。

2.1 质控摘要i以表格形式列出质控关键统计指标，包括总 reads 数、过滤后有效 reads 数、平均读长、最小/最大读长及 Q20/Q30 碱基占比等，帮助判断测序数据质量是否符合分析要求。

指标值
Number of reads42737.0
Total bases62168070.0 bp
Mean read length1454.67 bp
Min read length1205.0 bp
Max read length1647.0 bp
Median read length1455.0 bp
Read length N501455.0 bp
Mean read quality12.83
Median read quality12.76

2.2 质控图i以可视化图表展示测序 reads 的长度分布和质量分数分布特征，直观呈现数据质量状况。若峰形集中且偏向高质量区间，说明测序质量良好；若分布离散或存在大量低质量 reads，需谨慎解读下游分析结果。

QC plot

3. Alpha 多样性指标iAlpha 多样性量化单个样本内部的微生物群落多样性。Shannon 指数：综合考量物种丰富度和均匀度，越高表示多样性越丰富；Gini-Simpson/Inv_Simpson：反映优势物种的统治程度；Pielou Evenness：衡量各物种丰度分布的均匀程度（值域 0–1，越接近 1 越均匀）；Berger-Parker Dominance：最高丰度物种占总丰度的比例，反映群落优势度。

Sample Observed_Species Shannon Simpson_D Gini_Simpson Inv_Simpson Pielou_Evenness Berger_Parker_Dominance
demo 76 2.660563 0.101357 0.898643 9.866140 0.614345 0.187239

4. 物种组成饼图（Top 20 + Other）i展示样本中相对丰度最高的前 N 个物种及其占比，其余物种合并为 Other。扇区面积正比于相对丰度，帮助直观了解优势菌群构成。颜色经过优化确保相邻物种配色可区分；标注自动避让，避免重叠遮挡。

species pie chart
说明: 饼图使用不重复颜色；比例在图外侧显示并自动避让，避免重叠。

5. Top 物种丰度表i以表格形式列出丰度最高的前 N 个物种，包含物种名称（Species）、相对丰度（RelativeAbundance）及百分比（Percentage）等字段，支持下载 CSV 后在 Excel 或 R/Python 中进行进一步分析。

Species RelativeAbundance Percentage
Escherichia_coli 0.187239 18.72%
Bacillus_spizizenii 0.142516 14.25%
Listeria_monocytogenes 0.119459 11.95%
Enterococcus_faecalis 0.093023 9.30%
Limosilactobacillus_fermentum 0.090241 9.02%
Staphylococcus_sp_MZ7 0.072948 7.29%
Bacillus_sp_PS65 0.069171 6.92%
Staphylococcus_aureus 0.053667 5.37%
Shigella_flexneri 0.024448 2.44%
Pseudomonas_aeruginosa 0.017492 1.75%
Bacillus_sp_MD-5 0.016696 1.67%
Salmonella_enterica 0.013914 1.39%
Bacillus_sp_SG20033 0.011529 1.15%
Bacillus_sp_LJBS06 0.010733 1.07%
Staphylococcus_sp_T93 0.009740 0.97%
Bacillus_cabrialesii 0.005565 0.56%
Bacillus_tequilensis 0.005565 0.56%
Pseudomonas_sp_FDAARGOS_761 0.005367 0.54%
Listeria_swaminathanii 0.004969 0.50%
Staphylococcus_sp_MZ3 0.003975 0.40%
Other 0.041741 4.17%

6. 分类层级桑基图（Top 20 Species）i以交互式桑基流程图展示物种从高级分类阶元到物种水平的归属关系，节点宽度正比于对应类群的相对丰度。可直观追踪门（Phylum）→纲（Class）→目（Order）→科（Family）→属（Genus）→种（Species）各级的丰度流向，帮助理解群落的分类层级组成。

桑基图已内嵌到当前报告，不依赖外部 html 文件路径。

7. 原始鉴定结果预览（前100行）i展示鉴定工具输出的 final.tsv 文件前 100 行原始数据，包含每一条序列的完整分类注释和相对丰度。可用于核查鉴定结果细节或筛查低可信度的注释条目。完整数据请下载原始 final.tsv 文件。

Id Taxonomy RelativeAbundance
ASV_1 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Enterobacterales; f__Enterobacteriaceae; g__Escherichia_Shigella; s__Escherichia_coli 0.187239
ASV_2 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_spizizenii 0.142516
ASV_3 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Listeriaceae; g__Listeria; s__Listeria_monocytogenes 0.119459
ASV_4 d__Bacteria; p__Bacillota; c__Bacilli; o__Lactobacillales; f__Enterococcaceae; g__Enterococcus; s__Enterococcus_faecalis 0.093023
ASV_5 d__Bacteria; p__Bacillota; c__Bacilli; o__Lactobacillales; f__Lactobacillaceae; g__Limosilactobacillus; s__Limosilactobacillus_fermentum 0.090241
ASV_6 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Staphylococcaceae; g__Staphylococcus; s__Staphylococcus_sp_MZ7 0.072948
ASV_7 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_sp_PS65 0.069171
ASV_8 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Staphylococcaceae; g__Staphylococcus; s__Staphylococcus_aureus 0.053667
ASV_9 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Enterobacterales; f__Enterobacteriaceae; g__Escherichia_Shigella; s__Shigella_flexneri 0.024448
ASV_10 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Pseudomonadales; f__Pseudomonadaceae; g__Pseudomonas; s__Pseudomonas_aeruginosa 0.017492
ASV_11 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_sp_MD-5 0.016696
ASV_12 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Enterobacterales; f__Enterobacteriaceae; g__Salmonella; s__Salmonella_enterica 0.013914
ASV_13 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_sp_SG20033 0.011529
ASV_14 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_sp_LJBS06 0.010733
ASV_15 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Staphylococcaceae; g__Staphylococcus; s__Staphylococcus_sp_T93 0.009740
ASV_16 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_tequilensis 0.005565
ASV_17 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_cabrialesii 0.005565
ASV_18 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Pseudomonadales; f__Pseudomonadaceae; g__Pseudomonas; s__Pseudomonas_sp_FDAARGOS_761 0.005367
ASV_19 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Listeriaceae; g__Listeria; s__Listeria_swaminathanii 0.004969
ASV_20 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_sp_B28 0.003975
ASV_21 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Staphylococcaceae; g__Staphylococcus; s__Staphylococcus_sp_MZ3 0.003975
ASV_22 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_sp_FSL 0.003975
ASV_23 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Staphylococcaceae; g__Staphylococcus; s__Staphylococcus_sp_MZ8 0.003578
ASV_24 d__Bacteria; p__Mycoplasmatota; c__Mollicutes; o__Mycoplasmoidales; f__Metamycoplasmataceae; g__Mesomycoplasma; s__Mesomycoplasma_ovipneumoniae 0.002982
ASV_25 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Staphylococcaceae; g__Staphylococcus; s__Staphylococcus_sp_MZ9 0.002584
ASV_26 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Listeriaceae; g__Listeria; s__Listeria_ivanovii 0.002186
ASV_27 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Pseudomonadales; f__Pseudomonadaceae; g__Pseudomonas; s__Metapseudomonas_lalkuanensis 0.001988
ASV_28 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Cytobacillus; s__Cytobacillus_spongiae 0.001590
ASV_29 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_subtilis 0.001391
ASV_30 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Pseudomonadales; f__Pseudomonadaceae; g__Pseudomonas; s__Metapseudomonas_boanensis 0.001391
ASV_31 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Enterobacterales; f__Enterobacteriaceae; g__Mangrovibacter; s__Mangrovibacter_phragmitis 0.001193
ASV_32 d__Bacteria; p__Bacillota; c__Bacilli; o__Lactobacillales; f__Enterococcaceae; g__Enterococcus; s__Enterococcus_rotai 0.001193
ASV_33 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_sp_MT 0.000795
ASV_34 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Listeriaceae; g__Listeria; s__Listeria_grayi 0.000795
ASV_35 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Pseudomonadales; f__Pseudomonadaceae; g__Pseudomonas; s__Metapseudomonas_resinovorans 0.000596
ASV_36 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Pseudomonadales; f__Pseudomonadaceae; g__Pseudomonas; s__Pseudomonas_sp_ABC1 0.000596
ASV_37 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Pseudomonadales; f__Pseudomonadaceae; g__Pseudomonas; s__Metapseudomonas_furukawaii 0.000596
ASV_38 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_sp_BC1-43 0.000596
ASV_39 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_velezensis 0.000398
ASV_40 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Listeriaceae; g__Listeria; s__Listeria_seeligeri 0.000398
ASV_41 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Priestia; s__Priestia_filamentosa 0.000398
ASV_42 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Oceanospirillales; f__Balneatricaceae; g__Pokkaliibacter; s__Pokkaliibacter_plantistimulans 0.000398
ASV_43 d__Bacteria; p__Pseudomonadota; c__Alphaproteobacteria; o__Rhodobacterales; f__Paracoccaceae; g__Tabrizicola; s__Tabrizicola_piscis 0.000398
ASV_44 d__Bacteria; p__Bacillota; c__Bacilli; o__Lactobacillales; f__Streptococcaceae; g__Lactococcus; s__Listeria_seeligeri 0.000398
ASV_45 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_atrophaeus 0.000398
ASV_46 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_vallismortis 0.000398
ASV_47 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Pseudomonadales; f__Pseudomonadaceae; g__Pseudomonas; s__Pseudomonas_marincola 0.000398
ASV_48 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Staphylococcaceae; g__Staphylococcus; s__Staphylococcus_simiae 0.000398
ASV_49 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_rugosus 0.000199
ASV_50 d__Bacteria; p__Acidobacteriota; c__Holophagae; o__Acanthopleuribacterales; f__Acanthopleuribacteraceae; g__Sulfidibacter; s__Sulfidibacter_corallicola 0.000199
ASV_51 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Alicyclobacillaceae; g__Effusibacillus; s__Effusibacillus_dendaii 0.000199
ASV_52 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Anaerobacillus; s__Anaerobacillus_isosaccharinicus 0.000199
ASV_53 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_amyloliquefaciens 0.000199
ASV_54 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_paralicheniformis 0.000199
ASV_55 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_inaquosorum 0.000199
ASV_56 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Staphylococcaceae; g__Staphylococcus; s__Staphylococcus_caprae 0.000199
ASV_57 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Staphylococcaceae; g__Staphylococcus; s__Staphylococcus_capitis 0.000199
ASV_58 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Staphylococcaceae; g__Staphylococcus; s__Staphylococcus_arlettae 0.000199
ASV_59 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Staphylococcaceae; g__Staphylococcus; s__Staphylococcus_schweitzeri 0.000199
ASV_60 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Bacillaceae; g__Bacillus; s__Bacillus_sp_WP8 0.000199
ASV_61 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Listeriaceae; g__Listeria; s__Listeria_welshimeri 0.000199
ASV_62 d__Bacteria; p__Bacillota; c__Bacilli; o__Caryophanales; f__Staphylococcaceae; g__Staphylococcus; s__Staphylococcus_simulans 0.000199
ASV_63 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Enterobacterales; f__Enterobacteriaceae; g__Escherichia_Shigella; s__Shigella_boydii 0.000199
ASV_64 d__Bacteria; p__Pseudomonadota; c__Alphaproteobacteria; o__; f__; g__; s__Candidatus_Fonsibacter_ubiquis 0.000199
ASV_65 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Enterobacterales; f__Enterobacteriaceae; g__; s__Ehrlichia_sp_JZT12 0.000199
ASV_66 d__Bacteria; p__Bacteroidota; c__Chitinophagia; o__Chitinophagales; f__Chitinophagaceae; g__Flavisolibacter; s__Flavisolibacter_tropicus 0.000199
ASV_67 d__Bacteria; p__Bacteroidota; c__Chitinophagia; o__Chitinophagales; f__Chitinophagaceae; g__Lacibacter; s__Lacibacter_sediminis 0.000199
ASV_68 d__Bacteria; p__Bacteroidota; c__Cytophagia; o__Cytophagales; f__Cyclobacteriaceae; g__Algoriphagus; s__Algoriphagus_sanaruensis 0.000199
ASV_69 d__Bacteria; p__Chloroflexota; c__Anaerolineae; o__Anaerolineales; f__Anaerolineaceae; g__Anaerolinea; s__Anaerolinea_thermophila 0.000199
ASV_70 d__Bacteria; p__Bacillota; c__Bacilli; o__Lactobacillales; f__Lactobacillaceae; g__Limosilactobacillus; s__Limosilactobacillus_gastricus 0.000199
ASV_71 d__Bacteria; p__Planctomycetota; c__Phycisphaerae; o__Phycisphaerales; f__Phycisphaeraceae; g__Mucisphaera; s__Mucisphaera_calidilacus 0.000199
ASV_72 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Pseudomonadales; f__Pseudomonadaceae; g__Pseudomonas; s__Aquipseudomonas_campi 0.000199
ASV_73 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Oceanospirillales; f__Endozoicomonadaceae; g__Endozoicomonas; s__Endozoicomonas_sp_Mp262 0.000199
ASV_74 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Enterobacterales; f__Enterobacteriaceae; g__Yokenella; s__Yokenella_regensburgei 0.000199
ASV_75 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Pseudomonadales; f__Pseudomonadaceae; g__Permianibacter; s__Permianibacter_aggregans 0.000199
ASV_76 d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Oceanospirillales; f__Oceanospirillales_incertae_sedis; g__Pseudohongiella; s__Pseudohongiella_spirulinae 0.000199
ASV_77 d__Bacteria; p__Verrucomicrobiota; c__Opitutia; o__Opitutales; f__Opitutaceae; g__Oleiharenicola; s__Oleiharenicola_sp_Vm1 0.000199
完整结果请查看 final.tsv 文件。